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STEMCELL Technologies Inc oligodendrocyte precursor cell differentiation medium
Inhibition of OPC myelination by bovine testicular hyaluronidase ( BTH ) is not correlated with PH 20 enzymatic activity. (A) Mature OL s produced by culturing of OPC s in <t>OPCDM</t> without further treatment; (B) OL s cultured in OPCDM with 40 μ g/ mL of BTH IV ‐S; (C) OL s cultured in OPCDM with 40 μ g/ mL heat‐inactivated BTH IV ‐S ( HI ‐ BTH IV ‐S); (D) OL s cultured in OPCDM with 40 μ g/ mL rHuPH 20. After incubation for 48–96 h, cultures were fixed and labeled for the OL lineage markers: PDGFR α (red, marker of OPC s), MBP (green, mature OL s), and DAPI (blue; cell nuclei). (All images: 20 × objective magnification with optical zoom.) (E) Quantitation of maturation state of cells by counting MBP ‐positive cells (% of control, in OPCDM , panel A) following specified treatments. BTH IV ‐S, but not HI ‐ BTH IV ‐S or rHuPH 20 is significantly different from control (*: P < 0.05, one‐way ANOVA ).
Oligodendrocyte Precursor Cell Differentiation Medium, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Inhibition of OPC myelination by bovine testicular hyaluronidase ( BTH ) is not correlated with PH 20 enzymatic activity. (A) Mature OL s produced by culturing of OPC s in OPCDM without further treatment; (B) OL s cultured in OPCDM with 40 μ g/ mL of BTH IV ‐S; (C) OL s cultured in OPCDM with 40 μ g/ mL heat‐inactivated BTH IV ‐S ( HI ‐ BTH IV ‐S); (D) OL s cultured in OPCDM with 40 μ g/ mL rHuPH 20. After incubation for 48–96 h, cultures were fixed and labeled for the OL lineage markers: PDGFR α (red, marker of OPC s), MBP (green, mature OL s), and DAPI (blue; cell nuclei). (All images: 20 × objective magnification with optical zoom.) (E) Quantitation of maturation state of cells by counting MBP ‐positive cells (% of control, in OPCDM , panel A) following specified treatments. BTH IV ‐S, but not HI ‐ BTH IV ‐S or rHuPH 20 is significantly different from control (*: P < 0.05, one‐way ANOVA ).

Journal: Annals of Clinical and Translational Neurology

Article Title: PH20 is not expressed in murine CNS and oligodendrocyte precursor cells

doi: 10.1002/acn3.393

Figure Lengend Snippet: Inhibition of OPC myelination by bovine testicular hyaluronidase ( BTH ) is not correlated with PH 20 enzymatic activity. (A) Mature OL s produced by culturing of OPC s in OPCDM without further treatment; (B) OL s cultured in OPCDM with 40 μ g/ mL of BTH IV ‐S; (C) OL s cultured in OPCDM with 40 μ g/ mL heat‐inactivated BTH IV ‐S ( HI ‐ BTH IV ‐S); (D) OL s cultured in OPCDM with 40 μ g/ mL rHuPH 20. After incubation for 48–96 h, cultures were fixed and labeled for the OL lineage markers: PDGFR α (red, marker of OPC s), MBP (green, mature OL s), and DAPI (blue; cell nuclei). (All images: 20 × objective magnification with optical zoom.) (E) Quantitation of maturation state of cells by counting MBP ‐positive cells (% of control, in OPCDM , panel A) following specified treatments. BTH IV ‐S, but not HI ‐ BTH IV ‐S or rHuPH 20 is significantly different from control (*: P < 0.05, one‐way ANOVA ).

Article Snippet: Oligodendrocyte precursor cell differentiation medium (OPCDM) , STEMCELL Technologies , DMEM/F12 (Life Technologies) Pen Strep 10% BSA 5 μ L of 3 mmol/L T3 500 μ L of 5 mg/mL NAC.

Techniques: Inhibition, Activity Assay, Produced, Cell Culture, Incubation, Labeling, Marker, Quantitation Assay, Control

bFGF and bovine testicular hyaluronidase ( BTH ) IV ‐S similarly inhibit OPC differentiation. Evaluation of OPC differentiation cultured in OPCDM was performed on cells coincubated with 40 μ g/ mL rHuPH 20, 40 μ g/ mL of BTH IV ‐S, or 10 ng/ mL human recombinant bFGF . Only conditions where bFGF and BTH IV ‐S are added to the culture medium but not rHuPH 20 displayed results significantly different from the control (*: P < 0.05, one−way ANOVA).

Journal: Annals of Clinical and Translational Neurology

Article Title: PH20 is not expressed in murine CNS and oligodendrocyte precursor cells

doi: 10.1002/acn3.393

Figure Lengend Snippet: bFGF and bovine testicular hyaluronidase ( BTH ) IV ‐S similarly inhibit OPC differentiation. Evaluation of OPC differentiation cultured in OPCDM was performed on cells coincubated with 40 μ g/ mL rHuPH 20, 40 μ g/ mL of BTH IV ‐S, or 10 ng/ mL human recombinant bFGF . Only conditions where bFGF and BTH IV ‐S are added to the culture medium but not rHuPH 20 displayed results significantly different from the control (*: P < 0.05, one−way ANOVA).

Article Snippet: Oligodendrocyte precursor cell differentiation medium (OPCDM) , STEMCELL Technologies , DMEM/F12 (Life Technologies) Pen Strep 10% BSA 5 μ L of 3 mmol/L T3 500 μ L of 5 mg/mL NAC.

Techniques: Cell Culture, Recombinant, Control

OL s produce HA which is digested by bovine testicular hyaluronidase ( BTH ) IV ‐S and rHuPH 20. OPC s were grown in OPCDM to produce mature OL s with or without specified treatments. Cells were fixed and labeled with bHABP . Alexa Fluor 488‐conjugated Streptavidin (green) was used to visualize HA . The cells were counterstained with DAPI (blue). (A) Cells in OPCDM without further treatment; (B) cells treated with 10 ng/ mL bFGF ; (C) cells treated with 40 μ g/ mL BTH IV ‐S; (D) cells treated with 40 μ g/ mL rHuPH 20. (All images: 20 × objective magnification with optical zoom.) (E) Concentration of HA in supernatant measured by ELISA.

Journal: Annals of Clinical and Translational Neurology

Article Title: PH20 is not expressed in murine CNS and oligodendrocyte precursor cells

doi: 10.1002/acn3.393

Figure Lengend Snippet: OL s produce HA which is digested by bovine testicular hyaluronidase ( BTH ) IV ‐S and rHuPH 20. OPC s were grown in OPCDM to produce mature OL s with or without specified treatments. Cells were fixed and labeled with bHABP . Alexa Fluor 488‐conjugated Streptavidin (green) was used to visualize HA . The cells were counterstained with DAPI (blue). (A) Cells in OPCDM without further treatment; (B) cells treated with 10 ng/ mL bFGF ; (C) cells treated with 40 μ g/ mL BTH IV ‐S; (D) cells treated with 40 μ g/ mL rHuPH 20. (All images: 20 × objective magnification with optical zoom.) (E) Concentration of HA in supernatant measured by ELISA.

Article Snippet: Oligodendrocyte precursor cell differentiation medium (OPCDM) , STEMCELL Technologies , DMEM/F12 (Life Technologies) Pen Strep 10% BSA 5 μ L of 3 mmol/L T3 500 μ L of 5 mg/mL NAC.

Techniques: Labeling, Concentration Assay, Enzyme-linked Immunosorbent Assay

Key cell culture media and supplements

Journal: Annals of Clinical and Translational Neurology

Article Title: PH20 is not expressed in murine CNS and oligodendrocyte precursor cells

doi: 10.1002/acn3.393

Figure Lengend Snippet: Key cell culture media and supplements

Article Snippet: Oligodendrocyte precursor cell differentiation medium (OPCDM) , STEMCELL Technologies , DMEM/F12 (Life Technologies) Pen Strep 10% BSA 5 μ L of 3 mmol/L T3 500 μ L of 5 mg/mL NAC.

Techniques: Cell Culture, Concentration Assay, Cell Differentiation, Recombinant

Treatments

Journal: Annals of Clinical and Translational Neurology

Article Title: PH20 is not expressed in murine CNS and oligodendrocyte precursor cells

doi: 10.1002/acn3.393

Figure Lengend Snippet: Treatments

Article Snippet: Oligodendrocyte precursor cell differentiation medium (OPCDM) , STEMCELL Technologies , DMEM/F12 (Life Technologies) Pen Strep 10% BSA 5 μ L of 3 mmol/L T3 500 μ L of 5 mg/mL NAC.

Techniques: Concentration Assay, Cell Differentiation, Control

Expression of Murine Spam1 and Neuronal Markers in Cultured Oligodendrocyte Precursor Cells, Astrocytes, and Neurons

Journal: Annals of Clinical and Translational Neurology

Article Title: PH20 is not expressed in murine CNS and oligodendrocyte precursor cells

doi: 10.1002/acn3.393

Figure Lengend Snippet: Expression of Murine Spam1 and Neuronal Markers in Cultured Oligodendrocyte Precursor Cells, Astrocytes, and Neurons

Article Snippet: Oligodendrocyte precursor cell differentiation medium (OPCDM) , STEMCELL Technologies , DMEM/F12 (Life Technologies) Pen Strep 10% BSA 5 μ L of 3 mmol/L T3 500 μ L of 5 mg/mL NAC.

Techniques: Expressing, Cell Culture